Proteomics at the speed of life.
ProteoPulse turns a drop of blood into quantified proteins in 30 minutes, then keeps measuring, with a new result every 15 minutes. Continuous LC-MS proteomics, built for research today and the bedside tomorrow.
Nucleate Technical Excellence Award, Nucleate Los Angeles, 2026 Activator program
Benefits of a ProteoPulse system
High throughput
Parallelized injection with dual-trap, single-column (DTSC) LC means no delay between samples and more than 90% instrument utilization.
Robust LC
Patented custom valves minimize the number of failure points and retain full functionality.
Original DTSC
Valve-integrated DTSC
Versatile liquid handling
The liquid handler automates:
- Sample resuspension
- Sample mixing
- Your application
- Parallel integrated proteolysis (PIP)
Parallel integrated proteolysis (PIP)
Parallelized LC plus integrated proteolysis: no dead time between samples, and a continuous stream of protein measurements.
Sample in
A single microliter of whole blood, from a finger prick or draw.
Integrated proteolysis
HTA protease digests the sample in minutes, inline.
Parallelized LC-MS
Dual-trap, single-column chromatography feeds the mass spectrometer without pause.
Continuous data
A streaming biomarker readout, updated with every cycle.
The continuous cycle
Relevant publications
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2026bioRxiv preprint
Joining Two Perspectives of the Proteome with the Trypsin+HTA Protease Workflow
Tryptic and HTA-protease peptides analyzed together in one LC-MS run. The combined workflow also identified 216 additional non-artifact modifications, with no added sample preparation or analysis time.
24% more proteinsidentified on average in neat plasma than with trypsin alone -
2025Anal. Chem. 97, 6418–6426
Toward Real-Time Proteomics: Blood to Biomarker Quantitation in under One Hour
Single-step HTA digestion paired with dual-trap LC-MS. Also measured the labile peptide hormones angiotensin I and II at 8-minute intervals, and found dozens of blood proteins that trypsin-based workflows miss.
160+ proteinsquantified from 1 µL of whole blood in under one hour -
2023Anal. Chem. 95(24), 9145–9150
High-Throughput Single-Cell Proteomic Analysis of Organ-Derived Heterogeneous Cell Populations by Nanoflow Dual-Trap Single-Column Liquid Chromatography
A nanoflow version of the architecture for single cells, built from standard commercial parts, stable over more than 1,700 injections.
96 cells / daywith over 1,000 proteins quantified in individual cardiomyocytes -
2022Anal. Chem. 94, 12452–12460
Parallelization with Dual-Trap Single-Column Configuration Maximizes Throughput of Proteomic Analysis
The dual-trap, single-column foundation: 300–400 blood proteins and over 4,000 cell-lysate proteins per 15-minute run.
90%instrument utilization at 15 minutes per sample
One platform, time-resolved proteomics everywhere
Measuring the proteome continuously opens applications a single snapshot can't reach.
Fundamental research
- Time-resolved 'omics
- Therapy screening
- Accessible to non-specialists
Process development
- ADME and toxicology
- Organ-on-a-chip
- Biopharma production
Physical performance
- Athletic monitoring
- Readiness assessment
- Exposure tracking
Critical care
- Cardiac monitoring
- Real-time biomarkers
- Bedside deployment
Let us design your LC-MS solution
Tell us about your samples, your throughput and what you need to measure, and we'll follow up with a configuration and a quote. We'll reply within a few working days.